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Julian Mironov
Julian Mironov

Dod ( 383 ) Mp4


Report number 22-01 was supported by the US Navy BUMED under work unit number 60504. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, the Department of Defense, or the US Government.




Dod ( 383 ) mp4



The study protocol was approved by the Naval Health Research Center Institutional Review Board in compliance with all applicable Federal regulations governing the protection of human subjects. Research data were derived from an approved Naval Health Research Center Institutional Review Board protocol, number NHRC.1999.0003.


This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.


I'm trying to send a group of .mp4 files first from a web browser client, then to express, then to flask. This code might look simple, but it has taken literally days to get this far, because nobody on the internet has posted an example of how to do this with large files (.mp4 video files), and to be honest, there are a lot of inconsistencies in using these libraries.


The Autel Evo 2 will support microSD cards up to 128GB and the Chinese drone maker recommends using a Class 10 or a UHS-1 card, such as the 32GB SanDisk Extreme or the 64GB A2/V30/U3/Class 10 SanDisk Extreme Pro.


Buy your next drone directly from manufacturers, such as DJI, Parrot, Yuneec or retailers like Adorama, Amazon, B&H, BestBuy, DroneNerds or eBay. By using our links, we will make a small commission at no additional cost to you. Thank you for helping DroneDJ grow!


Sponsorship dollars go directly into programming that supports service members and their families that give so much. You help us serve those who serve our country. Sponsorship dollars enhance MWR quality of life programs cre- ating lasting memories that service members and their fami- lies keep with them during long deployments and throughout their military life. Sponsorship dollars allow for a wider variety of programs and services for our military as well as facility enhancements.


Sponsorship allows you to directly engage with over 300,000 people in the military community we serve through one-on- one encounters at our events or through strategic advertising. Military families appreciate all that is done to support them and this is reflected in their loyalty as consumers. They show this appreciation for our corporate partners no matter where in the world the Navy sends them. Sponsorships that are accompanied by advertising in MWR fa- cilities allow our corporate partners to gain exposure for their brands and messages and provide a platform to showcase their goods and services throughout the year.


FLEET & FAMILY SUPPORT PROGRAM (FFSP) Fleet & Family Support provides unique programs and various services to active duty, activated Reservists, retirees, and family members that promote self-re- liance and family resilience.


AUTO SKILLS CENTERS Everything amateur mechanics need to keep their automobiles running smoothly at Auto Skills Centers. BOWLING CENTERS Recreational bowling, leagues, professional and fun tournaments, birthday parties and command parties! CABINS, COTTAGES & RV PARKS Recreational Lodging: RV sites, tent sites, cot- tages, cabins, townhomes and resort-style vacation rentals. CHILD & YOUTH PROGRAMS (CYP) Planned programs of activities for each age group to promote the intellectual, social, emotional and physical development of your child. COMMUNITY RECREATION Outdoor recreation, community events, dis- count tickets to attractions and vacation book- ings, leisure skills development, recreation equipment rentals and more. FITNESS, SPORTS & AQUATICS Mission essential programs optimize phys- ical, mental, social and emotional personal health. MWR hosts more than 20 virtual and in-person 5K races and challenges a year.


NAVY GOLD STAR PROGRAM Navy Gold Star liaisons coordinate ser- vices with other agencies to address comprehensive needs and resolve complex individual issues for surviving family members of deceased active duty Sailors.


Event Sponsorship Opportunities Companies have an amazing opportunity to showcase their business by forming a strategic partnership with the Navy Region Mid-Atlantic MWR Sponsorship Department. Packages may be customized to fit any corporation's needs. Sponsorship of virtual events is also available on multiple social media platforms. Contact Sponsorship for more information.


NAVY REGION MID-ATLANTIC The Navy is organized into 10 regions worldwide and a headquarters element that we call Commander, Naval Installations Command (CNIC). Navy Region Mid-Atlantic encompasses 14 installations across 20 states and serves over 127,000 active duty and Reserve military personnel and 293,000 retirees and family members. Our civilian workforce consists of approximately 95,000 Non- Appropriated Fund (NAF) and Civil Service (GS) personnel. Visit www.cnic.navy.mil for installation information.


The average military family enjoys a household income that is 80% higher than the U.S. average. Military families and veterans represent an incredibly diverse group that accounts for more than 25% of local state business.


MAXIMIZE YOUR ADVERTISING EXPOSURE IN MWR FACILITIES! Advertising opportunities are available to build brand recognition in some of the most highly- visible MWR facilities including Ticket & Travel offices, fitness centers, single Sailor recreation


The NAS Oceana Air Show has solidified a ground breaking partnership with the Virginia Beach City Public School System and the Chesapeake Public School System to create an "Outdoor STEM Laboratory" on an unprecedented scale. With over 8,000 fifth grade students from Virginia Beach and Chesapeake visiting in 2019, the NAS Oceana Air Show STEM Lab Learning


Day shattered the Guinness World Record for the largest educational field trip. The STEM Laboratory consists of hands-on, interactive displays by local businesses, museums, universities and the U.S. Navy that are presented to the fifth graders of the participating schools.


* The Department of the Navy does not endorse any company, sponsor or their products or services. (All advertising must include this disclaimer). BASE MOVIE THEATERS MWR provides affordable, first-run movies for Sailors and their families at theaters across the globe. Promote your company in our pre-feature collection of movie slides and videos. Color advertisements are shown on full-size digital cinema movie screens for 30 seconds. The Epicenter Theater, located onboard Naval Station Great Lakes, has a total seating capacity of 175 and averages 6,000 patrons per month. Movies are shown Tuesday through Sunday each week.


LED Digital Signage Advertising Package: Twelve at Seaview Lanes Bowling Center (Bldg. 656), one at Ney Hall Galley (Bldg. 292), one at John H. Chafee Fitness Center (Bldg. 1109), one at Navy Gateway Inns & Suites (Bldg. 1688) and one at NAVSTA Headquarters (Bldg. 690)


Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.


Studies of RBC substitutes for human use have continued since the 1980s7,8,9,10. The products with the greatest potential are hemoglobin (Hb)-based O2 carriers9,10. In the United States, poly(ethyleneglycol)-conjugated Hb11,12,13, polymerized Hb14, intramolecularly cross-linked Hb15,16 have been developed and examined in clinical trials7,8,9,10,17,18,19,20. Most of these artificial O2-carriers are designed to increase molecular size and to avoid renal excretion. However, transfusion of such heterogeneous molecules has raised the blood pressure in humans to a greater or lesser extent. Two mechanisms for this side effect are presumed to be (i) nitric oxide (NO: an endothelium-derived relaxing factor) scavenging by Hb diffused into the extravascular space and (ii) oversupply of O2 to vascular walls by facilitated diffusion8,17,18,19,20,21,22,23,24. Despite intensive efforts, no substitute has been able to satisfy all requirements for clinical use. For pet animals, polymerized bovine Hb (Oxyglobin) used to be commercially available for the treatment of anemic dogs in the United States and the United Kingdom25. Alayash et al. reported that this formulation contained nonpolymerized Hb of a substantial amount (37.8%)26. Some side effects have been reported, such as vomiting, anorexia, and pyrexia, as well as discoloration of skin, mucous membranes, urine, and (darkened) feces27.


Expressions of rCSA using Pichia yeast have not been reported in the relevant literature. We have now prepared rCSA using Pichia pastoris (GS115) in a gram quantity. Pichia yeast secretes the target protein into culture medium so that the accumulated protein can be harvested easily by centrifugation, followed by general chromatographic technique. Results showed that the ideal purification was achieved using a combination of affinity chromatography and anion exchange chromatography.


(A) SDS-PAGE, (B) Native-PAGE, (C) SEC profiles, (D) MALDI-TOF mass spectra, and (E) CD spectra of rCSA and CSA. All results demonstrated clearly that these proteins possess identical molecular weight and structure. Full-length gel images of (A,B) are presented in Supplementary Fig. S1(A,B).


The CD spectrum of the Hb-rCSA3 cluster coincided accurately with the sum of the Hb spectrum and a three-fold amplified rCSA spectrum (Fig. 4C). This result proved that the secondary structure of individual protein unit was not perturbed by the coupling and that the rCSA/Hb composition ratio is 3/1 (mol/mol). The isoelectric point of Hb-rCSA3 cluster (pI: 4.7) was extremely close to the value of rCSA (pI: 4.5) (Fig. 4D and S5B), which supports enfolding of the Hb core by rCSAs. The surface net charges of this cluster are satisfactorily negative to circulate in the bloodstream for a long period. It is also noteworthy that the molecular features of Hb-rCSA3 cluster are exactly the same as those of the Hb-CSA3 cluster, which was prepared using native CSA. 041b061a72


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